What isSIINFEKL The search query "ova peptide SIINFEKL" primarily indicates a need for information regarding the SIINFEKL peptide, a specific epitope derived from ovalbuminA SIINFEKL-Based System to Measure MHC Class I Antigen .... This peptide is a crucial tool in immunological research, particularly for stimulating ovalbumin-specific T cells and studying antigen presentation.
The SIINFEKL peptide, a well-defined octameric sequence derived from ovalbumin (OVA), plays a significant role in immunological studies.Description, This is aclass I (Kb)-restricted peptide epitope of OVA, an octameric peptide from ovalbumin presented by the class I MHC molecule, H-2Kb. This peptide, specifically the OVA 257-264 sequence, is recognized as a potent epitope that binds to the MHC Class I molecule H-2Kb. Its well-characterized nature makes it an invaluable tool for researchers investigating T cell responses, vaccine development, and antigen presentation mechanismsAmino acid substitutions in the SIINFEKL (OVA) peptide ....
SIINFEKL is an 8-amino acid peptide (Ser-Ile-Ile-Phe-Asn-Phe-Lys-Leu) that originates from the ovalbumin protein, a primary component of egg whites.OVA peptides Its significance lies in its ability to be presented by MHC Class I molecules, specifically H-2Kb in mice. This presentation is critical for the activation of CD8+ cytotoxic T lymphocytes (CTLs), which are essential for cellular immunityMolecular Formula. C45H74N10O ; Synonyms.SIINFEKL; OVA PEPTIDE (257-264); C45H74N10O13; Chicken Ovalbumin Peptide; POV-3659-PI ; Molecular Weight. 963.1 g/mol.. Due to this specific binding and T cell activation capability, SIINFEKL is routinely used in various research applications.
The primary utility of the SIINFEKL peptide in research is its role in stimulating ovalbumin-specific T cells. This makes it a standard reagent for:
* T Cell Activation Studies: Researchers use SIINFEKL to activate and study the behavior of ovalbumin-specific T cells, particularly CD8+ T cells. This includes assessing T cell proliferation, cytokine production (like IFN-$\gamma$), and cytotoxic activity.
* Vaccine Development: SIINFEKL serves as a model antigen to test the efficacy of new vaccine adjuvants and formulations. By observing the T cell response to SIINFEKL, scientists can gauge how well a potential vaccine candidate induces a cellular immune response.
* Antigen Presentation Assays: The peptide is instrumental in studying the processes of antigen processing and presentation by antigen-presenting cells (APCs), particularly the role of MHC Class I molecules like H-2Kb. Assays such as ELISPOT are commonly employed to quantify peptide epitope specificity and cytokine release.
* Allergy Models: In some contexts, SIINFEKL is used to stimulate allergic reactions in allergy models, helping researchers understand the mechanisms behind certain types of immune hypersensitivity.(SIINFEKL). Description This is a class I (Kb)-restrictedpeptideepitope ofOVA, an octamericpeptidefrom ovalbumin presented by the class I MHC m… More ...
* Developing Detection Tools: Monoclonal antibodies, such as the 25-D1.16 antibody, have been developed that specifically recognize the SIINFEKL peptide when it is bound to the H-2Kb molecule.Ovalbumin Epitope SIINFEKL Self-Assembles into a ... These antibodies are crucial for detecting and quantifying antigen presentation events.
While SIINFEKL is the most commonly used ovalbumin epitope for MHC Class I presentation, researchers also utilize variants and related peptides for specific experimental purposes. For instance, the OVA G4 peptide (SIIGFEKL) is a variant of SIINFEKL that may be used in modified studiesFormation and Kinetics of MHC Class I-Ovalbumin Peptide .... Additionally, other ovalbumin-derived peptides, such as those restricted by MHC Class II molecules (e.gAmino acid substitutions in the SIINFEKL (OVA) peptide ...., OVA323-339), are used to study different arms of the adaptive immune responseOvalbumin Epitope SIINFEKL Self-Assembles into a .... The availability of endotoxin-free versions of these peptides is crucial for ensuring reliable experimental resultsGeneration of both MHC class I‐ and class II‐restricted ....
SIINFEKL peptide and its variants are commercially available from various scientific supply companies. When acquiring these peptides, researchers often look for high purity and, in many cases, endotoxin-free formulations to avoid confounding experimental outcomes. The molecular formula for SIINFEKL is C$_{45}$H$_{74}$N$_{10}$O$_{13}$, with a molecular weight of approximately 963.1 g/mol. Proper storage, typically at low temperatures, is essential to maintain peptide integrity and activity.
In summary, the SIINFEKL peptide is a fundamental reagent in immunology, providing a standardized and reliable means to investigate cellular immunity, antigen presentation, and the development of novel immunotherapies and vaccines. Its specific interaction with MHC Class I molecules and its ability to elicit robust T cell responses solidify its position as a critical tool in the research laboratory.OVA G4 peptide
Join the newsletter to receive news, updates, new products and freebies in your inbox.